Online Monitoring & Control

YSI offers several products for on-line monitoring and control of key analytes in cell culture bioreactors. The original YSI 2700M offers a cost-effective, simple method for monitoring (and with the addition of a feed pump, control). YSI has collaborated with Groton, Flownamics, and DASGIP to provide solutions for your more complex monitoring and control applications. For more detailed descriptions of each system, please follow the links below.

2700 M On-line Biochemistry Analyzer
For processes that require frequent monitoring and regulation, on-line analysis allows you the freedom to sample automatically and adjust both nutrient and byproduct concentrations.

Couple the YSI 2730 Aseptic Monitoring & Control system with the 2700 SELECT and pull samples directly from a bioreactor, measure important nutrients and byproducts, and immediately control the replenishment of nutrients, if needed, while maintaining the sterility of the process. All system components are sterilizable.

2700 ARS & 7100 ARS
Innovative combinations of YSI analyzers and Groton Biosystems automated samplers provide around-the-clock online sampling for process development and production environments. The 2700 ARS and 7100 ARS improve your process knowledge while making more productive use of your time. Integrate up to four bioreactors in an online analytic system which provides sanitary sampling and automated cleaning.

YSI Flownamics
The Flownamics SEG-FLOW™ 804M coupled with the YSI 2700 SELECT™ Biochemistry Analyzer provides automated on-line analysis. The SEG-FLOW™ draws samples from 1 to 8 bioreactor vessels using the FISP® Cell-free Sampling Probe(s) and delivers each to the YSI 2700, or up to 4 analyzers in all. The SEG-FLOW™ system can provide feed control to each vessel. In the feed control mode, the YSI 2700 sends the measured result back to the SEG-FLOW™ and posts it in the Flow-Web™ software. The system then activates a feed pump (or an existing feed system) based on the measured result and target concentration. There are multiple outputs to report data from each vessel.

YSI Groton DASGIP
The YSI/Groton/DASGIP system provides a complete closed loop feedback control system by integrating the YSI 7100, the Groton Biosystems Automated Reactor Sample System, and the DASGIP bioreactor control system. The result is a powerful, automated online, real time feedback control system for mammalian cell culture.

A YSI 7100, which can measure up to 6 analytes simultaneously, and a Groton ARS-M, are connected to a DASGIP Parallel Bioreactor System (PBS) controlled by the DASGIP Control 4.0 OPC Edition Software (DCS). The DASGIP Parallel Bioreactor Systems can operate multiple bioreactors at the same time controlling pH, dissolved oxygen, temperature, agitation and liquid feeds independently for each reactor.

After the ARS-M input and output paths are primed, a sample is delivered to the YSI7100 for analysis of feedstock concentration (glucose, methanol) and other analytes. The YSI Biochemistry Analyzer then transmits the glucose concentration data to the Groton ARS-M server, where the data is stored. The DASGIP Control OPC client automatically polls for and retrieves new analytical data from the ARS-M OPC server. If the glucose concentration is lower than the set point, the DCS then responds by activating the appropriate system pump to deliver a calculated amount of glucose stock solution into the bioreactor to achieve the preset final concentration

The current system is capable of connecting to and sampling from eight bioreactors. It can deliver the sample to as many as four analytical instruments (such as YSI Biochemistry Analyzer).

For more detailed information, please click here to view a YSI/Groton/DASGIP Application Note.